The book's main goal is to fulfil the prerequisites for CMLT/DMLT and undergraduate courses. It does so in a detailed and symbolic manner, covering all the necessary themes. For ease of comprehension, several concepts have been discussed using straightforward language and few sentences. Additionally, it covers the most recent developments in biochemistry, including the glucometer, HbA/C test, and autoanalysers. The book is structured into two sections; the first portion covers fundamental concepts in analytical, physical, and organic chemistry. The second section covers the purpose and application of biochemistry as well as cell structure, electrolytes, bodily fluids, enzymes, metabolism, hormones, liver/renal function tests, carbohydrates, proteins, and lipids, among other topics.