Previous years? questions are an asset for students preparing for competitive examinations. The book is scripted in a precise manner considering the fact that the previous years? questions are not repeated but the concepts are. We have taken a systematic approach to screen out the previous years? questions. Then, we have identified the areas from where most of the questions are often asked. Finally, we have considered each of these selected questions as a template and subsequently framed two replicates (Replicate 1 and Replicate 2) with a similar concept as done in DNA replication. In this way, the entire previous years? questions of DBT-JRF Biotechnology (DBT-BET) are now replicated. This approach is adopted for all the units of DBT-JRF Biotechnology. This brand new concept of the book will offer a cluster of questions on the prescribed syllabus. The most significant part is to have a complete explanation of all the replicated questions. It is a true scholarly book for DBT-JRF preparation.