The volume was designed to provide research students investigators and technicians write-ups on laboratory methods in sufficient detail to enable the carrying out of the procedures satisfactorily. The coverage of the Handbook was pretty wide ranging from breeding & immunization of animals preparation of monospecific polyvalent antisera monoclonal antibodies purification of antibodies linking of fluorescine & enzymes ligands to antibodies immunoelectrophoresis isoelectric focusing radioimmunoassays enzyme immunoassays separation analysis & culture of T B lymphocytes and monocytes cytotoxicity assays and immunocytochemistry. Chapters on clinical immunology hypersensitivity allergic reactions HLA typing complement system immune complexes and immunohematology were also included to be of utility for clinical immunologic investigations.