I have written this new edition based on INC Syllabus and basic requirement of nursing students. This book is written primarily for nursing students with intention of giving subject in simple language. The content are formulated and organized as per INC syllabus, so this book will be a complete guide for nursing students. I have added two sections, Section A contains ?Applied Microbiology? and Section B contains ?Infection Control & Safety?. Each chapter is provided in an Overview which explains about each chapter. End of the each chapter, I have given important questions. Diagrams and tables were given to understand key details of the topic. I have enclosed short answers at the end of the book which will help students to prepare for examination.